Paintable Bioactive Extracellular Vesicle Ink for Wound Healing

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Paintable Bioactive Extracellular Vesicle Ink for Wound Healing
Title:
Paintable Bioactive Extracellular Vesicle Ink for Wound Healing
Journal Title:
ACS Applied Materials & Interfaces
Publication Date:
19 May 2023
Citation:
Li, L., Wang, Z., Wang, K., Fu, S., Li, D., Wang, M., Cao, Y., Zhu, H., Li, Z., Weng, L., Li, Z., Ding, X., & Wang, L. (2023). Paintable Bioactive Extracellular Vesicle Ink for Wound Healing. ACS Applied Materials & Interfaces, 15(21), 25427–25436. https://doi.org/10.1021/acsami.3c03630
Abstract:
The treatment of cutaneous wounds involving complex biological processes has become a significant public health concern worldwide. Here, we developed an efficient extracellular vesicle (EV) ink to regulate the inflammatory microenvironment and promote vascular regeneration for wound healing. The technology, termed portable bioactive ink for tissue healing (PAINT), leverages bioactive M2 macrophage-derived EVs (EVM2) and a sodium alginate precursor, forming a biocompatible EV-Gel within 3 min after mixing, enabling it to be smeared on wounds in situ to meet diverse morphologies. The bioactive EVM2 reprogram macrophage polarization and promote the proliferation and migration of endothelial cells, thereby effectively regulating inflammation and enhancing angiogenesis in wounds. Through integration with a 3D printing pen, the platform enables EV-Gel to be applied to wound sites having arbitrary shapes and sizes with geometric matches for tissue repairment. When evaluated using a mouse wound model, PAINT technology accelerates cutaneous wound healing by promoting the angiogenesis of endothelial cells and the polarization of macrophages to M2 phenotype in vivo, demonstrating the high potential of bioactive EV ink as a portable biomedical platform for healthcare.
License type:
Publisher Copyright
Funding Info:
This work was supported by the Leading-edge Technology Programme of Jiangsu Natural Science Foundation (BK20212012), Natural Science Foundation (22207056, 62288102) and the Natural Science Foundation of Jiangsu Province (BK20210580). This work was also supported by the CAS Key Laboratory of Nano-Bio Interface (21NBI01) and Key Laboratory of Nanodevices and Applications (22ZS06)
Description:
“This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see doi.org/10.1021/acsami.3c03630
ISSN:
1944-8244
1944-8252
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