Paintable Bioactive Extracellular Vesicle Ink for Wound Healing

Paintable Bioactive Extracellular Vesicle Ink for Wound Healing

ACS Applied Materials & Interfaces

10.1021/acsami.3c03630

http://dx.doi.org/10.1021/acsami.3c03630

Li Li, Zhiyu Wang, Kepeng Wang, Siyuan Fu, Dan Li, Mao Wang, Yi Cao, Houjuan Zhu, Ziyan Li, Lixing Weng, Zhiyang Li, Xianguang Ding, Lianhui Wang

General Materials Science

19 May 2023

Li, L., Wang, Z., Wang, K., Fu, S., Li, D., Wang, M., Cao, Y., Zhu, H., Li, Z., Weng, L., Li, Z., Ding, X., & Wang, L. (2023). Paintable Bioactive Extracellular Vesicle Ink for Wound Healing. ACS Applied Materials & Interfaces, 15(21), 25427–25436. https://doi.org/10.1021/acsami.3c03630

The treatment of cutaneous wounds involving complex biological processes has become a significant public health concern worldwide. Here, we developed an efficient extracellular vesicle (EV) ink to regulate the inflammatory microenvironment and promote vascular regeneration for wound healing. The technology, termed portable bioactive ink for tissue healing (PAINT), leverages bioactive M2 macrophage-derived EVs (EVM2) and a sodium alginate precursor, forming a biocompatible EV-Gel within 3 min after mixing, enabling it to be smeared on wounds in situ to meet diverse morphologies. The bioactive EVM2 reprogram macrophage polarization and promote the proliferation and migration of endothelial cells, thereby effectively regulating inflammation and enhancing angiogenesis in wounds. Through integration with a 3D printing pen, the platform enables EV-Gel to be applied to wound sites having arbitrary shapes and sizes with geometric matches for tissue repairment. When evaluated using a mouse wound model, PAINT technology accelerates cutaneous wound healing by promoting the angiogenesis of endothelial cells and the polarization of macrophages to M2 phenotype in vivo, demonstrating the high potential of bioactive EV ink as a portable biomedical platform for healthcare.

Publisher Copyright

This work was supported by the Leading-edge Technology Programme of Jiangsu Natural Science Foundation (BK20212012), Natural Science Foundation (22207056, 62288102) and the Natural Science Foundation of Jiangsu Province (BK20210580). This work was also supported by the CAS Key Laboratory of Nano-Bio Interface (21NBI01) and Key Laboratory of Nanodevices and Applications (22ZS06)

“This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see doi.org/10.1021/acsami.3c03630

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Institute of Materials Research and Engineering