Peptide Amphiphile Mediated Co‐assembly for Nanoplasmonic Sensing

Peptide Amphiphile Mediated Co‐assembly for Nanoplasmonic Sensing

Angewandte Chemie International Edition

10.1002/anie.202214394

http://dx.doi.org/10.1002/anie.202214394

Zhicheng Jin, Yi Li, Ke Li, Jiajing Zhou, Justin Yeung, Chuxuan Ling, Wonjun Yim, Tengyu He, Yong Cheng, Ming Xu, Matthew N. Creyer, Yu‐Ci Chang, Pavla Fajtová, Maurice Retout, Baiyan Qi, Shuzhou Li, Anthony J. O'Donoghue, Jesse V. Jokerst

General Chemistry, Catalysis

21 November 2022

Jin, Z., Li, Y., Li, K., Zhou, J., Yeung, J., Ling, C., Yim, W., He, T., Cheng, Y., Xu, M., Creyer, M. N., Chang, Y., Fajtová, P., Retout, M., Qi, B., Li, S., O’Donoghue, A. J., & Jokerst, J. V. (2022). Peptide Amphiphile Mediated Co‐assembly for Nanoplasmonic Sensing. Angewandte Chemie International Edition, 62(4). Portico. https://doi.org/10.1002/anie.202214394

AbstractAromatic interactions are commonly involved in the assembly of naturally occurring building blocks, and these interactions can be replicated in an artificial setting to produce functional materials. Here we describe a colorimetric biosensor using co‐assembly experiments with plasmonic gold and surfactant‐like peptides (SLPs) spanning a wide range of aromatic residues, polar stretches, and interfacial affinities. The SLPs programmed in DDD−(ZZ)x−FFPC self‐assemble into higher‐order structures in response to a protease and subsequently modulate the colloidal dispersity of gold leading to a colorimetric readout. Results show the strong aggregation propensity of the FFPC tail without polar DDD head. The SLPs were specific to the target protease, i.e., Mpro, a biomarker for SARS‐CoV‐2. This system is a simple and visual tool that senses Mpro in phosphate buffer, exhaled breath condensate, and saliva with detection limits of 15.7, 20.8, and 26.1 nM, respectively. These results may have value in designing other protease testing methods.

Publisher Copyright

There was no specific funding for the research done

This is the peer reviewed version of the following article: Jin, Z., Li, Y., Li, K., Zhou, J., Yeung, J., Ling, C., Yim, W., He, T., Cheng, Y., Xu, M., Creyer, M. N., Chang, Y., Fajtová, P., Retout, M., Qi, B., Li, S., O’Donoghue, A. J., & Jokerst, J. V. (2022). Peptide Amphiphile Mediated Co‐assembly for Nanoplasmonic Sensing. Angewandte Chemie International Edition, 62(4). Portico. https://doi.org/10.1002/anie.202214394, which has been published in final form at doi.org/10.1002/anie.202214394. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited

https://oar.a-star.edu.sg/communities-collections/articles/19369

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Institute of Materials Research and Engineering