Lukito, B. R., Basri, N., Thong, A., Hermansen, C., Weingarten, M., & Peterson, E. C. (2023). Co-culture of Kluyveromyces marxianus and Meyerozyma guilliermondii with In Situ Product Recovery of 2-Phenylethanol. Journal of Agricultural and Food Chemistry, 71(23), 8991–8997. https://
doi.org/10.1021/acs.jafc.3c01356
Abstract:
Production of 2-phenylethanol (2-PE) via K. marxianus is well-established. However, co-culture with other microbes in combination with in situ product recovery (ISPR) yields improved selectivity and volumetric productivity. Fermentation of K. marxianus (MUCL 53775) with direct inclusion of absorptive polymer Hytrel®3548 achieved ISPR, but accumulation of the by-product phenylethyl acetate (PEA) was strongly favored. Co-culture of K. marxianus (MUCL 53775) with Meyerozyma guilliermondii (MUCL 28072) with ISPR limited PEA production, thereby improving 2-PE selectivity from 13% to 90%, compared to a pure culture of K. marxianus (MUCL 53775) under similar conditions. This improved volumetric productivity by 85% compared to 2-PE ISPR with a pure culture of K. marxianus. This is the first report of co-culture in a two-phase fermentation for 2-PE bioproduction and demonstrates that interactions between co-culture and ISPR techniques can modulate bioproduction between 2-PE and by-product PEA and this technique will be explored for other strain combinations and for other high-value molecules of interest.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research / project is supported by the A*STAR - Career Development Award (CDA)
Grant Reference no. : C210112029