Tan, J. H., Chen, A., Bi, J., Lim, Y. H., Wong, F. T., & Ow, D. S.-W. (2023). The Engineering, Expression, and Immobilization of Epimerases for D-allulose Production. International Journal of Molecular Sciences, 24(16), 12703. https://doi.org/10.3390/ijms241612703
Abstract:
The rare sugar D-allulose is a potential replacement for sucrose with a wide range of health benefits. Conventional production involves the employment of the Izumoring strategy, which utilises D-allulose 3-epimerase (DAEase) or D-psicose 3-epimerase (DPEase) to convert D-fructose into D-allulose. Additionally, the process can also utilise D-tagatose 3-epimerase (DTEase). However, the process is not efficient due to the poor thermotolerance of the enzymes and low conversion rates between the sugars. This review describes three newly identified DAEases that possess desirable properties for the industrial-scale manufacturing of D-allulose. Other methods used to enhance process efficiency include the engineering of DAEases for improved thermotolerance or acid resistance, the utilization of Bacillus subtilis for the biosynthesis of D-allulose, and the immobilization of DAEases to enhance its activity, half-life, and stability. All these research advancements improve the yield of D-allulose, hence closing the gap between the small-scale production and industrial-scale manufacturing of D-allulose.
License type:
Attribution 4.0 International (CC BY 4.0)
Funding Info:
This research is supported by core funding from: Agency for Science, Technology and Research (A*STAR)
Grant Reference no. : C211917006
This research is supported by core funding from: Agency for Science, Technology and Research (A*STAR)
Grant Reference no. : C211917010
This research / project is supported by the Singapore Food Agency - Singapore Food Story (SFS) R&D Programme
Grant Reference no. : W23W2D0009
This research / project is supported by the Singapore Food Agency - Singapore Food Story (SFS) R&D Programme
Grant Reference no. : H20H8a0003