Orthogonally crosslinked alginate conjugate thermogels with potential for cell encapsulation

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Orthogonally crosslinked alginate conjugate thermogels with potential for cell encapsulation
Title:
Orthogonally crosslinked alginate conjugate thermogels with potential for cell encapsulation
Journal Title:
Carbohydrate Polymers
Publication Date:
23 November 2022
Citation:
Ow, V., Chang, J. J., Chooi, W. H., Boo, Y. J., Tan, R. P. T., Wong, J. H. M., Parikh, B. H., Su, X., Ng, S. Y., Loh, X. J., & Xue, K. (2023). Orthogonally crosslinked alginate conjugate thermogels with potential for cell encapsulation. Carbohydrate Polymers, 302, 120308. https://doi.org/10.1016/j.carbpol.2022.120308
Abstract:
Hydrogels with more than one mode of crosslinking have gained interest due to improved control over hydrogel properties such as mechanical strength using multiple stimuli. In this work, sodium alginate was covalently conjugated onto thermoresponsive polyurethanes to prepare hybrid polymers (EPC-Alg) that are responsive to both temperature and Ca2+, forming orthogonally crosslinked hydrogels which are non-toxic to cells. Notably, the crosslinks are fully reversible, allowing for gel strength to be modulated via selective removal of either stimulus, or complete deconstruction of the hydrogel network by removing both stimuli. Higher alginate fractions increased the hydrophilicity and Ca2+ response of the EPC-Alg hydrogel, enabling tunable modulation of the thermal stability, stiffness and gelation temperatures. The EPC-Alg hydrogel could sustain protein release for a month and encapsulate neural spheroids with high cell viability after 7-day culture, demonstrating feasibility towards 3D cell encapsulation in cell-based biomedical applications such as cell encapsulation and cell therapy.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research / project is supported by the BMRC - HBMS IAF-PP
Grant Reference no. : H20C6a0033
Description:
ISSN:
0144-8617
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