Distinct skin microbiome community structures in congenital ichthyosis

Distinct skin microbiome community structures in congenital ichthyosis

British Journal of Dermatology

10.1111/bjd.21687

http://dx.doi.org/10.1111/bjd.21687

Khek‐Chian Tham, Rachel Lefferdink, Kaibo Duan, Seong Soo Lim, X.F. Colin C. Wong, Erin Ibler, Benedict Wu, Hajar Abu‐Zayed, Stephanie M. Rangel, Ester Del Duca, Mashkura Chowdhury, Margot Chima, Hee Jee Kim, Bernett Lee, Emma Guttman‐Yassky, Amy S. Paller, John E. A. Common

Dermatology

28 May 2022

Tham, K., Lefferdink, R., Duan, K., Lim, S. S., Wong, X. F. C. C., Ibler, E., Wu, B., Abu‐Zayed, H., Rangel, S. M., Del Duca, E., Chowdhury, M., Chima, M., Kim, H. J., Lee, B., Guttman‐Yassky, E., Paller, A. S., & Common, J. E. A. (2022). Distinct skin microbiome community structures in congenital ichthyosis. British Journal of Dermatology, 187(4), 557–570. Portico. https://doi.org/10.1111/bjd.21687

Background: The ichthyoses are rare genetic keratinizing disorders that share the characteristics of an impaired epidermal barrier and increased risk of microbial infections. Although ichthyotic diseases share a T helper (Th) 17 cell immune signature, including increased expression of antimicrobial peptides, the skin microbiota of ichthyoses is virtually unexplored. Objectives: To analyse the metagenome profile of skin microbiome for major congenital ichthyosis subtypes. Methods: Body site-matched skin surface samples were collected from the scalp, upper arm and upper buttocks of 16 healthy control participants and 22 adult patients with congenital forms of ichthyosis for whole metagenomics sequencing analysis. Results: Taxonomic profiling showed significant shifts in bacteria and fungi abundance and sporadic viral increases across ichthyosis subtypes. Cutibacterium acnes and Malassezia were significantly reduced across body sites, consistent with skin barrier disruption and depletion of lipids. Microbial richness was reduced, with specific increases in Staphylococcus and Corynebacterium genera, as well as shifts in fungal species, including Malassezia. Malassezia globosa was reduced at all body sites, whereas M. sympodialis was reduced in the ichthyotic upper arm and upper buttocks. Malassezia slooffiae, by contrast, was strikingly increased at all body sites in participants with congenital ichthyosiform erythroderma (CIE) and lamellar ichthyosis (LI). A previously undescribed Trichophyton species was also detected as sporadically colonizing the skin of patients with CIE, LI and epidermolytic ichthyosis subtypes. Conclusions: The ichthyosis skin microbiome is significantly altered from healthy skin with specific changes predominating among ichthyosis subtypes. Skewing towards the Th17 pathway may represent a response to the altered microbial colonization in ichthyosis. What is already known about this topic? The skin microbiome of congenital ichthyoses is largely unexplored. Microbes play an important role in pathogenesis, as infections are common. The relative abundances of staphylococci and corynebacteria is increased in the cutaneous microbiome of patients with Netherton syndrome, but extension of these abundances to all congenital ichthyoses is unexplored. What does this study add? A common skin microbiome signature was observed across congenital ichthyoses. Distinct microbiome features were associated with ichthyosis subtypes. Changes in microbiome may contribute to T helper 17 cell immune polarization. What is the translational message? These data provide the basis for comparison of the microbiome with lipidomic and transcriptomic alterations in these forms of ichthyosis and consideration of correcting the dysbiosis as a therapeutic intervention.

Publisher Copyright

This research is supported by core funding from: A*STAR - BMRC EDB IAF-PP
Grant Reference no. : H17/01/a0/004

This research is supported by core funding from: BMRC EDB IAF-PP
Grant Reference no. : H18/01a0/016

This research is supported by core funding from: BMRC IAF
Grant Reference no. : 311006

This research is supported by core funding from: BMRC IAF-PP
Grant Reference no. : H19/01/a0/024

This research is supported by core funding from: BMRC transition funds
Grant Reference no. : H16/99/b0/011

This research / project is supported by the National Research Foundation (NRF) - Shared Infrastructure Support
Grant Reference no. : NRF SIS NRF2017_SISFP09

Funded as an Investigator-initiated grant from Novartis 26 CAIN457AUS05T (ASP, EG-Y)

This is the peer reviewed version of the following article: Tham, K., Lefferdink, R., Duan, K., Lim, S. S., Wong, X. F. C. C., Ibler, E., Wu, B., Abu‐Zayed, H., Rangel, S. M., Del Duca, E., Chowdhury, M., Chima, M., Kim, H. J., Lee, B., Guttman‐Yassky, E., Paller, A. S., & Common, J. E. A. (2022). Distinct skin microbiome community structures in congenital ichthyosis. British Journal of Dermatology, 187(4), 557–570. Portico. https://doi.org/10.1111/bjd.21687, which has been published in final form at doi.org/10.1111/bjd.21687. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited

https://oar.a-star.edu.sg/communities-collections/articles/18839

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