Lee, G., Ko, Y.-G., Bae, K. H., Kurisawa, M., Kwon, O. K., & Kwon, O. H. (2022). Green tea catechin-grafted silk fibroin hydrogels with reactive oxygen species scavenging activity for wound healing applications. Biomaterials Research, 26(1). https://doi.org/10.1186/s40824-022-00304-3
Abstract:
Abstract
Background
Overproduction of reactive oxygen species (ROS) is known to delay wound healing by causing oxidative tissue damage and inflammation. The green tea catechin, (–)-Epigallocatechin-3-O-gallate (EGCG), has drawn a great deal of interest due to its strong ROS scavenging and anti-inflammatory activities. In this study, we developed EGCG-grafted silk fibroin hydrogels as a potential wound dressing material.
Methods
The introduction of EGCG to water-soluble silk fibroin (SF-WS) was accomplished by the nucleophilic addition reaction between lysine residues in silk proteins and EGCG quinone at mild basic pH. The resulting SF-EGCG conjugate was co-crosslinked with tyramine-substituted SF (SF-T) via horseradish peroxidase (HRP)/H2O2 mediated enzymatic reaction to form SF-T/SF-EGCG hydrogels with series of composition ratios.
Results
Interestingly, SF-T70/SF-EGCG30 hydrogels exhibited rapid in situ gelation (< 30 s), similar storage modulus to human skin (≈ 1000 Pa) and superior wound healing performance over SF-T hydrogels and a commercial DuoDERM® gel dressings in a rat model of full thickness skin defect.
Conclusion
This study will provide useful insights into a rational design of ROS scavenging biomaterials for wound healing applications.
License type:
Attribution 4.0 International (CC BY 4.0)
Funding Info:
This research is supported by core funding from: IBB
Grant Reference no. : NIL
This work was supported by the National Research Foundation of Korea (NRF) grants (2020R1I1A3072735) and the Grand Information Technology Research Center Support Program (IITP-2022-2020-0-01612) through the Institute of Information & Communications Technology Planning & Evaluation (IITP) funded by the Ministry of Science and ICT (MSIT), Korea.