CRISPR-Cas strategies for natural product discovery and engineering in actinomycetes

Page view(s)
625
Checked on Aug 03, 2024
CRISPR-Cas strategies for natural product discovery and engineering in actinomycetes
Title:
CRISPR-Cas strategies for natural product discovery and engineering in actinomycetes
Journal Title:
Process Biochemistry
Publication Date:
16 January 2021
Citation:
Heng, E., Tan, L. L., Zhang, M. M., & Wong, F. T. (2021). CRISPR-Cas strategies for natural product discovery and engineering in actinomycetes. Process Biochemistry, 102, 261–268. https://doi.org/10.1016/j.procbio.2021.01.007
Abstract:
Fuelled by CRISPR-Cas technology, a synergistic combination of advances in genomics, synthetic biology and bioinformatics ushered in an era of genome-guided natural product discovery and engineering. As a versatile programmable DNA targeting tool, CRISPR-Cas is increasingly employed to accelerate host and pathway engineering of actinomycetes, which are prolific producers of bioactive natural products. Here we discuss the current state of the art, challenges and opportunities of CRISPR-Cas strategies to discover, diversify and improve the production of natural products from actinomycetes. Special emphasis is placed on the rapidly expanding CRISPR-Cas toolboxes for genome editing of non-Streptomyces actinomycetes.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research / project is supported by the National Research Foundation - Competitive Research Program
Grant Reference no. : NRF-CRP19-2017-05
Description:
ISSN:
1359-5113
Files uploaded:

File Size Format Action
process-biochemistry-jan-submission.pdf 718.53 KB PDF Open