Lim, H. Y. G., Kostic, L., & Barker, N. (2022). Organoid systems for recapitulating the intestinal stem cell niche and modeling disease in vitro. Advances in Stem Cells and Their Niches, 57–96. https://doi.org/10.1016/bs.asn.2021.10.001
The maintenance and repair of the native intestinal epithelium is sustained by niche signals that direct the self-renewal and differentiation of stem cells residing at intestinal crypt bases. These signals originate from other differentiated epithelial cells, the surrounding mesenchymal stroma, nerve and immune cells, gut microbiota, and the underlying extracellular matrix. Efforts to recapitulate the morphology, self-renewal capacity, and functions of the intestinal epithelium in vitro have therefore sought to incorporate these diverse cellular and microbial elements of the stem cell niche together with engineering approaches to model the physical and molecular features of the extracellular matrix. Ultimately, such models will enable us to generate key insights into epithelial homeostasis, regeneration, and intestinal diseases within a near-physiological in vitro setting, and serve as a powerful platform to establish therapeutic approaches for the treatment of intestinal disorders.
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This research / project is supported by the National Medical Research Council - Open Fund - Individual Research Grant (OF-IRG)
Grant Reference no. : OFIRG19may-0069
This research / project is supported by the National Medical Research Council - NRF Investigatorship
Grant Reference no. : NRF12017-03