Ma, P., Chen, Y., Lai, X., Zheng, J., Ye, E., Loh, X. J., … Li, Z. (2021). The Translational Application of Hydrogel for Organoid Technology: Challenges and Future Perspectives. Macromolecular Bioscience, 21(10), 2100191. doi:10.1002/mabi.202100191
Human organoids mimic the physiology and tissue architecture of organs and is of great significance for promoting the study of human diseases. Traditionally, organoid cultures rely predominantly on animal or tumor-derived extracellular matrix (ECM), resulting in poor reproducibility. This limits their utility in for large-scale drug screening and application for regenerative medicine. Recently, synthetic polymeric hydrogels, with high biocompatibility and biodegradability, stability, uniformity of compositions, and high throughput properties, have emerged as potential materials for achieving three-dimensional (3D) architectures for organoid cultures. Compared to conventional animal or tumour-derived organoids, these newly engineered hydrogel-based organoids more closely resemble human organs, as they are able to mimic native structural and functional properties observed in-situ. In this review, we will summarise recent developments in hydrogel-based organoid culture, highlight emergent hydrogel technology and discuss future challenges in applying them to organoid culture.
This research is supported by core funding from: Institute of Materials Research and Engineering, A*STAR
Grant Reference no. : Grant number is not applicable
National Key R&D Program of China (Grant No. 2020YFA0908100), the Natural Science Foundation of China (81773661)
This is the peer reviewed version of the following article: Ma, P., Chen, Y., Lai, X., Zheng, J., Ye, E., Loh, X. J., … Li, Z. (2021). The Translational Application of Hydrogel for Organoid Technology: Challenges and Future Perspectives. Macromolecular Bioscience, 21(10), 2100191. doi:10.1002/mabi.202100191, which has been published in final form at http://dx.doi.org/10.1002/mabi.202100191. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions