Thermal proximity coaggregation for system-wide profiling of protein complex dynamics in cells

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Thermal proximity coaggregation for system-wide profiling of protein complex dynamics in cells
Title:
Thermal proximity coaggregation for system-wide profiling of protein complex dynamics in cells
Other Titles:
Science
Publication Date:
09 March 2018
Citation:
Tan, C. S. H., Go, K. D., Bisteau, X., Dai, L., Yong, C. H., Prabhu, N., … Nordlund, P. (2018). Thermal proximity coaggregation for system-wide profiling of protein complex dynamics in cells. Science, 359(6380), 1170–1177. doi:10.1126/science.aan0346
Abstract:
Proteins differentially interact with each other across cellular states and conditions, but an efficient proteome-wide strategy to monitor them is lacking.We report the application of thermal proximity coaggregation (TPCA) for high-throughput intracellular monitoring of protein complex dynamics. Significant TPCA signatures observed among well-validated protein-protein interactions correlate positively with interaction stoichiometry and are statistically observable in more than 350 annotated human protein complexes. Using TPCA, we identified many complexes without detectable differential protein expression, including chromatin-associated complexes, modulated in S phase of the cell cycle. Comparison of six cell lines by TPCA revealed cell-specific interactions even in fundamental cellular processes. TPCA constitutes an approach for system-wide studies of protein complexes in nonengineered cells and tissues and might be used to identify protein complexes that are modulated in diseases.
License type:
Publisher Copyright
Funding Info:
This research / project is supported by the BMRC, A*STAR - Young Investigator Grant (1610151038)
Grant Reference no. : 1610151038

This research / project is supported by the NRF - NRF-CRP
Grant Reference no. : NRF2016NRF-CRP001-024
Description:
This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on Vol. 359 dated 9 Mar 2018, 10.1126/science.aan0346
ISSN:
0036-8075
1095-9203
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