Generation of Micronuclei and Detection of Chromosome Pulverization

Generation of Micronuclei and Detection of Chromosome Pulverization

Methods in Molecular Biology

10.1007/978-1-4939-7780-2_12

https://doi.org/10.1007/978-1-4939-7780-2_12

Monique N. H. Luijten, Jeannie X. T. Lee, Sixun Chen, Karen C. Crasta

22 March 2018

Luijten M.N.H., Lee J.X.T., Chen S., Crasta K.C. (2018) Generation of Micronuclei and Detection of Chromosome Pulverization. In: Pellestor F. (eds) Chromothripsis. Methods in Molecular Biology, vol 1769. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7780-2_12

Lagging chromosomes that arise after chromosome mis-segregation during cell division can be encapsulated within small structures known as micronuclei. A link between whole-chromosome mis-segregation and chromothripsis has been demonstrated via micronuclear chromosome pulverization. Here, we describe methods to efficiently generate micronuclei and examine downstream cell fates, specifically with regard to DNA damage and chromosome pulverization.

Publisher Copyright

There was no specific funding for the research done

https://oar.a-star.edu.sg/communities-collections/articles/17538

978-1-4939-7779-6
978-1-4939-7780-2

Institute of Molecular and Cell Biology