Emi2 Is Essential for Mouse Spermatogenesis

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Emi2 Is Essential for Mouse Spermatogenesis
Emi2 Is Essential for Mouse Spermatogenesis
Journal Title:
Cell Reports
Publication Date:
18 July 2017
Gopinathan, L., Szmyd, R., Low, D., Diril, M. K., Chang, H.-Y., Coppola, V., … Kaldis, P. (2017). Emi2 Is Essential for Mouse Spermatogenesis. Cell Reports, 20(3), 697–708. doi:10.1016/j.celrep.2017.06.033
The meiotic functions of Emi2, an inhibitor of the APC/C complex, have been best characterized in oocytes where it mediates metaphase II arrest as a component of the cytostatic factor. We generated knockout mice to determine the in vivo functions of Emi2—in particular, its functions in the testis, where Emi2 is expressed at high levels. Male and female Emi2 knockout mice are viable but sterile, indicating that Emi2 is essential for meiosis but dispensable for embryonic development and mitotic cell divisions. We found that, besides regulating cell-cycle arrest in mouse eggs, Emi2 is essential for meiosis I progression in spermatocytes. In the absence of Emi2, spermatocytes arrest in early diplotene of prophase I. This arrest is associated with decreased Cdk1 activity and was partially rescued by a knockin mouse model of elevated Cdk1 activity. Additionally, we detected expression of Emi2 in spermatids and sperm, suggesting potential post-meiotic functions for Emi2.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research is supported by core funding from: A*STAR, Biomedical Research Council
Grant Reference no. :