Circulating Salivary miRNA hsa-miR-221 as Clinically Validated Diagnostic Marker for Hand, Foot, and Mouth Disease in Pediatric Patients

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Circulating Salivary miRNA hsa-miR-221 as Clinically Validated Diagnostic Marker for Hand, Foot, and Mouth Disease in Pediatric Patients
Title:
Circulating Salivary miRNA hsa-miR-221 as Clinically Validated Diagnostic Marker for Hand, Foot, and Mouth Disease in Pediatric Patients
Journal Title:
EBioMedicine
Keywords:
Publication Date:
04 May 2018
Citation:
Min, Nyo et al. EBioMedicine, Volume 31, 299 - 30
Abstract:
Enhancements in the diagnostic capabilities using host biomarkers are currently much needed where sensitivity and specificity issues plague the diagnosis of Hand, Foot and Mouth Disease (HFMD) in pediatrics clinical samples. We investigated miRNome profiles of HFMD saliva samples against healthy children and developed miRNA-based diagnosis models. Our 6-miRNA scoring model predicted HFMD with an overall accuracy of 85.11% in the training set and 92.86% in the blinded test set of Singapore cohort. Blinded evaluation of the model in Taiwan HFMD cases resulted in 77.08% accuracy with the 6-miRNA model and 68.75% with the 4-miRNA model. The strongest predictor of HFMD in all of the panels, hsa-miR-221 was found to be consistently and significantly downregulated in all of our HFMD cohorts. This is the first study to prove that HFMD infection could be diagnosed by circulating miRNAs in patient's saliva. Moreover, this study also serves as a stepping stone towards the future development of other infectious disease diagnosis workflows using novel biomarkers.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
J.J.H.C. was supported by the MINDEF DRIP Grant [ R571-000-210-232 ]; and the CBRG grant [ CBRG13nov02 ] from National Medical Research Council, Ministry of Health, Singapore). R.W. was supported by grants from the Ministry of Science and Technology, Taiwan [ MOST-106-2320-B-182-007 ]; and Chang Gung Memorial Hospital Research Fund [ CMRPD1G0021 , CMRPD1A0193 , CMRPD1A0553 , CMRPD1F0281-2 and CMPRD1E0411-3 ].
Description:
ISSN:
2352-3964
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