STING-dependent translation inhibition restricts RNA virus replication

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STING-dependent translation inhibition restricts RNA virus replication
STING-dependent translation inhibition restricts RNA virus replication
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Proceedings of the National Academy of Sciences
Publication Date:
12 February 2018
STING regulates translation Kate M. Franz, William J. Neidermyer, Yee-Joo Tan, Sean P. J. Whelan, Jonathan C. Kagan Proceedings of the National Academy of Sciences Feb 2018, 115 (9) E2058-E2067; DOI: 10.1073/pnas.1716937115
In mammalian cells, IFN responses that occur during RNA and DNA virus infections are activated by distinct signaling pathways. The RIG-I–like-receptors (RLRs) bind viral RNA and engage the adaptor MAVS (mitochondrial antiviral signaling) to promote IFN expression, whereas cGAS (cGMP–AMP synthase) binds viral DNA and activates an analogous pathway via the protein STING (stimulator of IFN genes). In this study, we confirm that STING is not necessary to induce IFN expression during RNA virus infection but also find that STING is required to restrict the replication of diverse RNA viruses. The antiviral activities of STING were not linked to its ability to regulate basal expression of IFN-stimulated genes, activate transcription, or autophagy. Using vesicular stomatitis virus as a model, we identified a requirement of STING to inhibit translation during infection and upon transfection of synthetic RLR ligands. This inhibition occurs at the level of translation initiation and restricts the production of viral and host proteins. The inability to restrict translation rendered STING-deficient cells 100 times more likely to support productive viral infections than wild-type counterparts. Genetic analysis linked RNA sensing by RLRs to STING-dependent translation inhibition, independent of MAVS. Thus, STING has dual functions in host defense, regulating protein synthesis to prevent RNA virus infection and regulating IFN expression to restrict DNA viruses.
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Funding Info:
J.C.K. is supported by NIH Grants AI093589, AI116550, and P30 DK34854 and an Investigators in the Pathogenesis of Infectious Disease Award from the Burroughs Wellcome Fund. K.M.F. is supported by the Harvard Herchel Smith Graduate Fellowship.
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