Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation

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Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation
Title:
Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation
Other Titles:
Nature Communications
Keywords:
Publication Date:
23 May 2018
Citation:
Ezzoukhry, Z., Henriet, E., Cordelières, F.P. et al. Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation. Nat Commun 9, 2031 (2018). https://doi.org/10.1038/s41467-018-04461-9
Abstract:
Invadosomes are F-actin-based structures involved in extracellular matrix degradation, cell invasion, and metastasis formation. Analyzing their proteome is crucial to decipher their molecular composition, to understand their mechanisms, and to find specific elements to target them. However, the specific analysis of invadosomes is challenging, because it is difficult to maintain their integrity during isolation. In addition, classical purification methods often suffer from contaminations, which may impair data validation. To ensure the specific identification of invadosome components, we here develop a method that combines laser microdissection and mass spectrometry, enabling the analysis of subcellular structures in their native state based on low amounts of input material. Using this combinatorial method, we show that invadosomes contain specific components of the translational machinery, in addition to known marker proteins. Moreover, functional validation reveals that protein translation activity is an inherent property of invadosomes, which is required to maintain invadosome structure and activity.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
E.H. is supported by a PhD from the Ministère de l’Enseignement Supérieur et de la Recherche. Z.E. was supported by a fellowship from ANR-13-JJC-JSV1-0005. This work has been supported by grants from ANR-13-JJC-JSV1-0005, SIRIC BRIO, La Ligue Nationale contre le Cancer. F.S. and V.M. are supported by fundings from Equipe Labellisée, Ligue Nationale contre le Cancer 2016, SIRIC BRIO, and INCA, PLBIO15-135 (to F.S.) and PLBIO2014-182 (to V.M.). L.M. is supported by an INSERM/Région Alsace Ph.D fellowship, followed by a 1-year support from ARC. The work performed in the team of J.G. is funded by the French National Cancer Institute (INCa, PLBIO15-135)
Description:
ISSN:
2041-1723
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