Ding, C., Lim, Y.C., Chia, S.Y. et al. De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis. Nat Commun 9, 1329 (2018). https://doi.org/10.1038/s41467-018-03754-3
Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development.
This work was supported by Singapore NRF fellowship (NRF-2011NRF-NRFF 001-025), Tanoto Initiative in Diabetes Research to L.S., National Medical Research Council’s Cooperative Basic Research Grant (CBRG; NMRC/CBRG/0070/2014 and NMRC/CBRG/0101/2016), Open Fund-Individual Research (OF-IRG) Grant (NMRC/OFIRG/0062/2017), and Ministry of Education (MOE) Tier2 grant (MOE2017-T2-2-009). This work was supported by the RNA Biology Center at CSI Singapore, NUS, from funding by the Singapore Ministry of Education’s Tier 3 grants, grant number MOE2014-T3-1-006. This work was also supported by the Recruitment Program for Young Professionals (C.D.), Zhejiang Key Subject of Medical Science (C.D.), National Natural Science Foundation of China (81700770), and Zhejiang Provincial Natural Science Foundation of China (LY18C060006).