Fate mapping via Ms4a3 expression history traces monocyte-derived cells

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Fate mapping via Ms4a3 expression history traces monocyte-derived cells
Title:
Fate mapping via Ms4a3 expression history traces monocyte-derived cells
Journal Title:
Cell
Keywords:
Publication Date:
05 September 2019
Citation:
Liu Z, Gu Y, Chakarov S, Bleriot C, Kwok I, Chen X, Shin A, Huang W, Dress RJ, Dutertre CA, Schlitzer A, Chen J, Ng LG, Wang H, Liu Z, Su B, Ginhoux F. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells. Cell. 2019 Sep 5;178(6):1509-1525.e19. doi: 10.1016/j.cell.2019.08.009. PMID: 31491389.
Abstract:
Most tissue-resident macrophage (RTM) populations are seeded by waves of embryonic hematopoiesis and are self-maintained independently of a bone marrow contribution during adulthood. A proportion of RTMs, however, is constantly replaced by blood monocytes, and their functions compared to embryonic RTMs remain unclear. The kinetics and extent of the contribution of circulating monocytes to RTM replacement during homeostasis, inflammation, and disease are highly debated. Here, we identified Ms4a3 as a specific gene expressed by granulocyte-monocyte progenitors (GMPs) and subsequently generated Ms4a3TdT reporter, Ms4a3Cre, and Ms4a3CreERT2 fate-mapping models. These models traced efficiently monocytes and granulocytes, but no lymphocytes or tissue dendritic cells. Using these models, we precisely quantified the contribution of monocytes to the RTM pool during homeostasis and inflammation. The unambiguous identification of monocyte-derived cells will permit future studies of their function under any condition.
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Funding Info:
This research is supported by the National Research Foundation, under its NRF-NRFI2017-02 project.
Description:
Please find the link to the article at the publisher's URL: https://doi.org/10.1016/j.cell.2019.08.009
ISSN:
0092-8674
1097-4172
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