Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators

Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators
Title:
Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators
Other Titles:
PLOS One
Keywords:
Publication Date:
01 August 2017
Citation:
Article Source: Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators Bai Z, Chai Xr, Yoon MJ, Kim HJ, LO KA, et al. (2017) Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators. PLOS Biology 15(8): e2002176. https://doi.org/10.1371/journal.pbio.2002176
Abstract:
Enhancing brown fat activity and promoting white fat browning are attractive therapeutic strategies for treating obesity and associated metabolic disorders. To provide a comprehensive picture of the gene regulatory network in these processes, we conducted a series of transcriptome studies by RNA sequencing (RNA-seq) and quantified the mRNA and long noncoding RNA (lncRNA) changes during white fat browning (chronic cold exposure, beta-adrenergic agonist treatment, and intense exercise) and brown fat activation or inactivation (acute cold exposure or thermoneutrality, respectively). mRNA–lncRNA coexpression networks revealed dynamically regulated lncRNAs to be largely embedded in nutrient and energy metabolism pathways. We identified a brown adipose tissue–enriched lncRNA, lncBATE10, that was governed by the cAMP-cAMP response element-binding protein (Creb) axis and required for a full brown fat differentiation and white fat browning program. Mechanistically, lncBATE10 can decoy Celf1 from Pgc1α, thereby protecting Pgc1α mRNA from repression by Celf1. Together, these studies provide a comprehensive data framework to interrogate the transcriptomic changes accompanying energy homeostasis transition in adipose tissue.
License type:
http://creativecommons.org/licenses/by-nd/4.0/
Funding Info:
National Medical Research Council http://www.nmrc.gov.sg/content/nmrc_internet/home.html (grant number NMRC/CBRG/0101/2016). Received by SUN L. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Medical Research Council http://www.nmrc.gov.sg/content/nmrc_internet/home.html (grant number NMRC/CBRG/0070/2014). Received by SUN L and Chen P. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Singapore Ministry of Education https://www.moe.gov.sg/ (grant number MOE2014-T3-1-006). Supported by by the RNA Biology Center at CSI Singapore, NUS, from funding by the Singapore Ministry of Education’s Tier 3 grants, received by SUN L. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Singapore NRF fellowship https://www.nrf.gov.sg/funding-grants/nrf-fellowship-and-nrf-investigatorship (grant number NRF-2011NRF-NRFF 001-025). Received by SUN L. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Description:
ISSN:
1544-9173
1545-7885
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