Kim B, Fukuda M, Lee JY, Su D, Sanu S, Silvin A, Khoo ATT, Kwon T, Liu X, Chi W, Liu X, Choi S, Wan DSY, Park SJ, Kim JS, Ginhoux F, Je HS, Chang YT. Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate. Angew Chem Int Ed Engl. 2019 Jun 11;58(24):7972-7976. doi: 10.1002/anie.201903058. Epub 2019 Apr 30. PMID: 31038839.
Abstract:
Microglia, the brain‐resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure–activity relationships study, we developed CDr20, a high‐performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome‐scale CRISPR‐Cas9 knockout screen, the UDP‐glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
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Funding Info:
This research is supported by the National Research Foundation, under its NRF-NRFI2017-02 project.
Description:
This is the peer reviewed version of the following article: Kim B, Fukuda M, Lee JY, Su D, Sanu S, Silvin A, Khoo ATT, Kwon T, Liu X, Chi W, Liu X, Choi S, Wan DSY, Park SJ, Kim JS, Ginhoux F, Je HS, Chang YT. Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate. Angew Chem Int Ed Engl. 2019 Jun 11;58(24):7972-7976. doi: 10.1002/anie.201903058. Epub 2019 Apr 30. PMID: 31038839, which has been published in final form at https://doi.org/10.1002/anie.201903058. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.