Probing the Molecular Determinants of Fluorinase Specificity

Probing the Molecular Determinants of Fluorinase Specificity
Title:
Probing the Molecular Determinants of Fluorinase Specificity
Other Titles:
Chemical Communications
Publication Date:
27 January 2017
Citation:
Chem.Commun.,2017,53, 2559
Abstract:
Molecular determinants of FlA1 fluorinase specificity were probed using50-chloro-50-deoxyadenosine (50-ClDA) analogs as substrates and FlA1active site mutants. Modifications at F213 or A279 residues are beneficialtowards these modified substrates, including 50-chloro-50-deoxy-2-ethynyladenosine, ClDEA (410-fold activity improvement), andconferred novel activity towards substrates not readily acceptedby wild-type FlA1.
License type:
http://creativecommons.org/licenses/by-nc/4.0/
Funding Info:
Funded by GlaxoSmithKline – SingaporeEconomic Development Board Partnership for Green and Sustain-able Manufacturing and A*STAR ICES
Description:
Article is open access
ISBN:

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