Metabolic Engineering Strategies for Sustainable Terpenoid Flavor and Fragrance Synthesis

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Metabolic Engineering Strategies for Sustainable Terpenoid Flavor and Fragrance Synthesis
Title:
Metabolic Engineering Strategies for Sustainable Terpenoid Flavor and Fragrance Synthesis
Journal Title:
Journal of Agricultural and Food Chemistry
Publication Date:
23 December 2019
Citation:
Abstract:
Terpenoids derived from plant material are widely applied in the flavor and fragrance industry. Traditional extraction methods are unsustainable, but microbial synthesis offers a promising solution to attain efficient production of natural-identical terpenoids. Overproduction of terpenoids in microbes requires careful balancing of the synthesis pathway constituents within the constraints of host cell metabolism. Advances in metabolic engineering have greatly facilitated overcoming the challenges of achieving high titers, rates, and yields TRYs). The review summarizes recent development in the molecular biology toolbox to achieve high TRYs for terpenoid biosynthesis, mainly in the two industrial platform microorganisms: Escherichia coli and Saccharomyces cerevisiae. The biosynthetic pathways, including alternative pathway designs, are briefly introduced, followed by recently developed methodologies used for pathway, genome, and strain optimization. Integrated applications of these tools are important to achieve high “TRYs” of terpenoid production and pave the way for translating laboratory research into successful commercial manufacturing.
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Funding Info:
This research is supported by the BioTransformation Innovation Platform, A*STAR, under its IAFPP programme (award no H1701a0006)
Description:
“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Journal of Agricultural and Food Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [10.1021/acs.jafc.9b06203, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].”
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