Ang, J., Lee, Y.‐A., Raghothaman, D., Jayaraman, P., Teo, K.L., Khan, F.J., Reuveny, S., Chang, Y.‐T., Kang, N.‐Y. and Oh, S. (2019), Rapid Detection of Senescent Mesenchymal Stromal Cells by a Fluorescent Probe. Biotechnol. J., 14: 1800691. doi:10.1002/biot.201800691
Abstract:
Despite intense interest in human mesenchymal stromal cells (MSCs), monitoring of the progressive occurrence of senescence has been hindered by the lack of efficient detection tools. Here, the discovery of a novel MSC senescence-specific fluorescent probe (CyBC9) identified by a high-throughput screen is reported. Compared with the prototypical senescence-associated β-galactosidase (SA-β-gal) staining, the CyBC9 assay is rapid (2 h) and nontoxic and can thus be applied to live cells. It is shown that CyBC9 is able to stain early and late senescent populations both in monolayer- and in microcarrier-based cultures. Finally, to investigate the mechanism of CyBC9, colocalization assays are performed and it is found that CyBC9 is accumulated in the mitochondria of senescent MSCs presumably due to the loss of membrane potential. Taken together, it is expected that CyBC9 will be a useful tool to ameliorate cell therapy through rapid and early screening of senescent phenotypes in clinically relevant MSCs.
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Funding Info:
This research is supported by the A*STAR Joint Council Office, under its Development Programme (DP) Grant (Award 1334k00083)
Description:
This is the peer reviewed version of the following article: Ang, J., Lee, Y.‐A., Raghothaman, D., Jayaraman, P., Teo, K.L., Khan, F.J., Reuveny, S., Chang, Y.‐T., Kang, N.‐Y. and Oh, S. (2019), Rapid Detection of Senescent Mesenchymal Stromal Cells by a Fluorescent Probe. Biotechnol. J., 14: 1800691. doi:10.1002/biot.201800691
, which has been published in final form at https://doi.org/10.1002/biot.201800691. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.