Wenn-Chyau Lee, Renia Laurent; Microscopy-based Methods for Rosetting Assay in Malaria Research; Vol 10, Iss 13, July 05, 2020
Abstract:
In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly by facilitating microvasculature occlusion along with direct endothelial cytoadherence by the infected erythrocytes. There are different microscopy-based techniques to visualize rosettes but neither of these techniques has yet to qualify as the official “gold standard” method. We have found that these techniques can be used interchangeably, provided that the conditions of the experiments are properly controlled. Here, we presented three methods as options for rosetting assay, i.e., the unstained wet mount technique, acridine orange based-fluorescence microscopy technique and Giemsa stained wet mount method, with preparation steps that enable consistent performance in rosetting experiments
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
LR was supported by core funding from A*STAR to SIgN. LR were also funded by A*STAR grant (JCO-DP BMSI/15-800006-SIGN). WCL was funded by Open Fund- Young Individual Research Grant (OF-YIRG NMRC/OFYIRG/0070/2018) by the National Medical Research Council, Ministry of Health, Singapore.
Description:
open access journal; www.bio-protocol.org/e3665 Copyright for authors
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