Ottenheim, C., Meier, K., Zimmermann, W., & Wu, J. C. (2014). Isolation of Filamentous Fungi Exhibiting High Endoxylanase Activity in Lignocellulose Hydrolysate. Applied Biochemistry and Biotechnology, 175(4), 2066–2074. https://doi.org/10.1007/s12010-014-1427-8
Abstract:
For complete degradation of hemicellulose into its monomers from lignocellulose biomass, the synergistic action of a broad range of hydrolytic enzymes is needed. Therefore, production of enzymes from their natural producer is desirable. To obtain a powerful β-1,4-endoxylanase producing fungus, 304 environmental samples were collected from various locations in Singapore, leading to 603 isolates. Among them, 71 exhibiting β-1,4-endoxylanase activity were identified belonging mainly to the genera of Aspergillus, Penicillium, and Trichoderma. Further analysis revealed Aspergillus niger DSM 26641 as a potential and stable β-1,4-endoxylanase producer, being able to grow in hydrothermal lignocellulose hydrolysate exhibiting its maximal β-1,4-endoxylanase activity at pH 4 and 60 °C. This strain is thought to be very suitable for lactic acid production in a simultaneous saccharification and fermentation at pH values below 5.
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Funding Info:
Science and Engineer-ing Research Council (SERC grant no. 0921590133, SERC grant no. 1124004027), A*STAR Research Attachment Program (ARAP)
Description:
This is a post-peer-review, pre-copyedit version of an article published in Applied Biochemistry and Biotechnology. The final authenticated version is available online at: https://doi.org/10.1007/s12010-014-1427-8