Identification and engineering of 32 membered antifungal macrolactone notonesomycins

Identification and engineering of 32 membered antifungal macrolactone notonesomycins
Title:
Identification and engineering of 32 membered antifungal macrolactone notonesomycins
Other Titles:
Microbial Cell Factories
Publication Date:
19 March 2020
Citation:
Goh, F., Zhang, M.M., Lim, T.R. et al. Identification and engineering of 32 membered antifungal macrolactone notonesomycins. Microb Cell Fact 19, 71 (2020). https://doi.org/10.1186/s12934-020-01328-x
Abstract:
Notonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. aminophilus DSM 40186. In a high throughput antifungal screening campaign, we identified an alternative notonesomycin A producing strain, Streptomyces sp. A793, and its biosynthetic gene cluster. From this strain, we further characterized a new more potent antifungal non-sulfated analogue, named notonesomycin B. Through CRISPR–Cas9 engineering of the biosynthetic gene cluster, we were able to increase the production yield of notonesomycin B by up to 18-fold as well as generate a strain that exclusively produces this analogue.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
This research is supported by core funding from the Bioinformatics Institute, ARES. WLY, EH, MMZ, FTW are supported by National Research Foundation, Singapore [NRF2013-THE001-094].
Description:
ISSN:
1475-2859
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