Transient conformational modification of immunoglobulin G duringpurification by protein A affinity chromatography

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Transient conformational modification of immunoglobulin G duringpurification by protein A affinity chromatography
Title:
Transient conformational modification of immunoglobulin G duringpurification by protein A affinity chromatography
Journal Title:
Journal of Chromatography A
Publication Date:
04 April 2015
Citation:
Gagnon P, Nian R, Leong D, Hoi A. Transient conformational modification of immunoglobulin G during purification by protein A affinity chromatography. J Chromatogr A. 2015;1395:136‐142. doi:10.1016/j.chroma.2015.03.080
Abstract:
Exposure of three native IgG1 monoclonal antibodies to 100mM acetate, pH 3.5 had no significant effect on their hydrodynamic size (11.5±0.5nm), while elution from protein A with the same buffer created a conformation of 5.5±1.0nm. Formation of the reduced-size conformation was preceded by the known destabilization of the second constant domain of the heavy chain (Cγ2) by contact with protein A, then compounded by exposure to low pH, creating extended flexibility in the hinge-Cγ2 region and allowing the Fab region to fold over the Fc region. The reduced-size conformation was necessary for complete elution. It persisted unchanged for at least 7 days under elution conditions. Physiological conditions restored native size, and it was maintained on re-exposure to 100mM acetate, pH 3.5. Protein A-mediated destabilization and subsequent restoration of native size did not create aggregates, but the reduced-size conformation was more susceptible to aggregation by secondary stress than native antibody. Protein A-mediated formation of the reduced-size conformation is probably universal during purification of human IgG1 antibodies, and may occur with other subclasses and IgG from other species, as well as Fc-fusion proteins.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
This research / project is supported by the Exploit Technologies Pte Ltd, A*STAR, under its ETPL GAP-Funded (Project No. ETPL/12-R15GAP-0009).
Description:
ISSN:
1873-3778
0021-9673
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