Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays

Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
Title:
Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
Other Titles:
Diagnostics
DOI:
10.3390/diagnostics10010011
Publication Date:
24 December 2019
Citation:
Tan, L.K.; Wong, W.Y.; Yang, H.T.; Huber, R.G.; Bond, P.J.; Ng, L.C.; Maurer-Stroh, S.; Hapuarachchi, H.C. Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays. Diagnostics 2020, 10, 11.
Abstract:
Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
This study was supported by the internal funds from the National Environment Agency (NEA), Singapore. S.M.-S. was partially supported by the A*STAR HEIDI programme (grant number: H1699f0013).
Description:
ISSN:
2075-4418
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