Monitoring structural modulation of redox-sensitive proteins in cells with MS-CETSA

Monitoring structural modulation of redox-sensitive proteins in cells with MS-CETSA
Monitoring structural modulation of redox-sensitive proteins in cells with MS-CETSA
Other Titles:
Redox Biology
Publication Date:
14 March 2019
Wendi Sun, Lingyun Dai, Han Yu, Brenda Puspita, Tianyun Zhao, Feng Li, Justin L. Tan, Yan Ting Lim, Ming Wei Chen, Radoslaw M. Sobota, Daniel G. Tenen, Nayana Prabhu, Pär Nordlund, Monitoring structural modulation of redox-sensitive proteins in cells with MS-CETSA, Redox Biology, Volume 24, 2019, 101168, ISSN 2213-2317,
Reactive oxygen species (ROS) induce different cellular stress responses but can also mediate cellular signaling. Augmented levels of ROS are associated with aging, cancer as well as various metabolic and neurological disorders. ROS can also affect the efficacy and adverse effects of drugs. Although proteins are key mediators of most ROS effects, direct studies of ROS-modulated-protein function in the cellular context are very challenging. Therefore the understanding of specific roles of different proteins in cellular ROS responses is still relatively rudimentary. In the present work we show that Mass Spectrometry-Cellular Thermal Shift Assay (MS-CETSA) can directly monitor ROS and redox modulations of protein structure at the proteome level. By altering ROS levels in cultured human hepatocellular carcinoma cell lysates and intact cells, we detected CETSA responses in many proteins known to be redox sensitive, and also revealed novel candidate ROS sensitive proteins. Studies in intact cells treated with hydrogen peroxide and sulfasalazine, a ROS modulating drug, identified not only proteins that are directly modified, but also proteins reporting on downstream cellular effects. Comprehensive changes are seen on rate-limiting proteins regulating key cellular processes, including known redox control systems, protein degradation, epigenetic control and protein translational processes. Interestingly, concerted shifts on ATP-binding proteins revealed redox-induced modulation of ATP levels, which likely control many cellular processes. Collectively, these studies establish CETSA as a novel method for cellular studies of redox modulations of proteins, which implicated in a wide range of processes and for the discovery of CETSA-based biomarkers reporting on the efficacy as well as adverse effects of drugs.
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We thank Drs. Chris Soon Heng Tan, Kelly Hew, Loo Chien Wang, Ying Yu Liang and Lars Abrahmsen for discussions, and the Protein Production Platform, Nanyang Technological University for recombinant protein preparation. P.N. acknowledges a startup grant from Nanyang Technological University and grants from the Swedish Research Council, the Swedish Cancer Society, Radiumhemmets funds and the Knut and Alice Wallenberg foundation. This research is also supported partly by the Singapore Ministry of Health’s National Medical Research Council, under its MOHIAFCAT2/004/2015 to P.N. and R.M.S, A*STAR BMRC YIG2015 grant to R.M.S. This research is also supported by the Singapore Ministry of Health's National Medical Research Council under its Singapore Translational Research (STaR) Investigator Award (to D.G.T.), and by the National Research Foundation Singapore and the Singapore Ministry of Education under its Research Centres of Excellence initiative.
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