Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

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Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2
Title:
Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2
Journal Title:
Stem Cell Research
Keywords:
Publication Date:
09 August 2019
Citation:
Generation of four H1 hESC sublines carrying a hemizygous knock-out/ mutant MECP2 Ruizhu Zenga, Harwin Sidika, Kim S. Robinson, Franklin L. Zhong, Bruno Reversade, Mahmoud A. Pouladi https://doi.org/10.1016/j.scr.2019.101533
Abstract:
Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
This work was funded by Strategic Positioning Fund for Genetic Orphan Diseases (SPF2012/005) and SUREKids (IAF311019) from the Agency for Science Technology and Research (Singapore) and TLGM-NDD from Agency for Science Technology and Research (Singapore) and the National University of Singapore.
Description:
ISSN:
1873-5061
1876-7753
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