Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women

Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women

Clinical and Translational Immunology

10.1002/cti2.1082

https://doi.org/10.1002/cti2.1082

Fok-Moon Lum, Vipin Narang, Susan Hue, Jie Chen, Naomi McGovern, Ravisankar Rajarethinam, Jeslin J. L. Tan, Siti Naqiah Amrun, Yi-Hao Chan, Cheryl Y. P. Lee, Tze-Kwang Chua, Wearn-Xin Yee, Nicholas K. W. Yeo, Thiam-Chye Tan, Xuan Liu, Sam Haldenby, Yee-Sin Leo, Florent Ginhoux, Jerry K. Y. Chan, Julian Hiscox, Chia-Yin Chong, Lisa F. P. Ng

05 November 2019

Lum, F.‐M., Narang, V., Hue, S., Chen, J., McGovern, N., Rajarethinam, R., Tan, J.J., Amrun, S.N., Chan, Y.‐H., Lee, C.Y., Chua, T.‐K., Yee, W.‐X., Yeo, N.K., Tan, T.‐C., Liu, X., Haldenby, S., Leo, Y.‐s., Ginhoux, F., Chan, J.K., Hiscox, J., Chong, C.‐Y. and Ng, L.F. (2019), Immunological observations and transcriptomic analysis of trimester‐specific full‐term placentas from three Zika virus‐infected women. Clin Transl Immunol, 8: e01082. doi:10.1002/cti2.1082

Objectives: Effects of Zika virus (ZIKV) infection on placental development during pregnancy are unclear. Methods: Full‐term placentas from three women, each infected with ZIKV during specific pregnancy trimesters, were harvested for anatomic, immunologic and transcriptomic analysis. Results: In this study, each woman exhibited a unique immune response with raised IL‐1RA, IP‐10, EGF and RANTES expression and neutrophil numbers during the acute infection phase. Although ZIKV NS3 antigens co‐localised to placental Hofbauer cells, the placentas showed no anatomic defects. Transcriptomic analysis of samples from the placentas revealed that infection during trimester 1 caused a disparate cellular response centred on differential eIF2 signalling, mitochondrial dysfunction and oxidative phosphorylation. Despite these, the babies were delivered without any congenital anomalies. Conclusion: These findings should translate to improve clinical prenatal screening procedures for virus‐infected pregnant patients.

http://creativecommons.org/licenses/by/4.0/

This work was supported by the Singapore Biomedical Research Council [BMRC; core research grants to the Singapore Immunology Network (SIgN)] and the BMRC and Singapore Agency for Science, Technology, and Research–led Zika Virus Consortium Fund (project 15/1/82/27/001). SIgN immuno-monitoring and flow cytometry platforms are supported by the Immuno-monitoring Service Platform (ISP) grant, NRF2017_SISFP09, and BMRC transition funds #H16/99/b0/011. This work was also partly funded by the National Institute for Health Research Health Protection Research Unit (NIHR HPRU) in Emerging and Zoonotic Infections at University of Liverpool in partnership with Public Health England (PHE), in collaboration with Liverpool School of Tropical Medicine.

https://oar.a-star.edu.sg/communities-collections/articles/15136

2050-0068

Singapore Immunology Network