DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method

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DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method
Title:
DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method
Other Titles:
Biochimie
Publication Date:
13 September 2017
Citation:
Ichiro Hirao, Michiko Kimoto, Kyung Hyun Lee, DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method, Biochimie, Volume 145, 2018, Pages 15-21, ISSN 0300-9084, https://doi.org/10.1016/j.biochi.2017.09.007.
Abstract:
A novel aptamer generation method to greatly augment the affinity and stability of DNA aptamers was developed by genetic alphabet expansion combined with mini-hairpin DNA technology. The genetic alphabet expansion increases the physicochemical and structural diversities of DNA aptamers by introducing extra components, unnatural bases, as a fifth base, allowing for the enhancement of DNA aptamer affinities. Furthermore, the mini-hairpin DNA technology stabilizes DNA aptamers against nuclease digestion and thermal denaturation, by introducing an extraordinarily stable mini-hairpin DNA containing a GCGAAGC sequence. This novel method provides stabilized high-affinity DNA aptamers for diagnostic and therapeutic applications.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
This work was supported by the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology and Research, Singapore) and the National Research Foundation of Singapore (NRF-CRP17-2017-07)..
Description:
ISSN:
0300-9084
1638-6183
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