Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants

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Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants
Title:
Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants
Other Titles:
Chemical Communications
Keywords:
Publication Date:
25 October 2017
Citation:
Chem. Commun., 2017,53, 12309-12312
Abstract:
Thermostable T7 RNA polymerase variants were explored for genetic alphabet expansion transcription involving the unnatural Ds‒Pa pair. One variant exhibited high incorporation efficiencies of functionally modified Pa substrates and enabled the simultaneous incorporation of 2ʹ-fluoro-nucleoside triphosphates of pyrimidines into transcripts, allowing the generation of novel, highly functional RNA molecules.
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PublisherCopyrights
Funding Info:
This work was supported by IBN (Biomedical Research Council, Agency for Science, A*STAR, Singapore) (to M.K. and I.H.), JST/PRESTO [JPMJPR13K9 to M.K.], NIH [5U54EB015403-02 to A.D.E.], Welch Foundation [F-1654 to A.D.E.], and National Security Science and Engineering Faculty Fellowship [FA9550-10-1-0169 to A.D.E.].
Description:
ISSN:
1359-7345
1364-548X
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