Dynamic proteome profiling of human pluripotent stem cellderived pancreatic progenitors

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Dynamic proteome profiling of human pluripotent stem cellderived pancreatic progenitors
Dynamic proteome profiling of human pluripotent stem cellderived pancreatic progenitors
Journal Title:
Stem Cells
Publication Date:
11 December 2019
Loo, LSW, Vethe, H, Soetedjo, AAP, et al. Dynamic proteome profiling of human pluripotent stem cell‐derived pancreatic progenitors. Stem Cells. 2020; 38: 542– 555. https://doi.org/10.1002/stem.3135
A comprehensive characterization of the molecular processes controlling cell fate decisions is essential to derive stable progenitors and terminally differentiated cells that are functional from human pluripotent stem cells (hPSCs). Here, we report the use of quantitative proteomics to describe early proteome adaptations during hPSC differentiation toward pancreatic progenitors. We report that the use of unbiased quantitative proteomics allows the simultaneous profiling of numerous proteins at multiple time points, and is a valuable tool to guide the discovery of signaling events and molecular signatures underlying cellular differentiation. We also monitored the activity level of pathways whose roles are pivotal in the early pancreas differentiation, including the Hippo signaling pathway. The quantitative proteomics data set provides insights into the dynamics of the global proteome during the transition of hPSCs from a pluripotent state toward pancreatic differentiation.
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Funding Info:
L.S.W.L. is supported by an A*STAR Graduate Scholarship. A.K.K.T. is supported by the Institute of Molecular and Cell Biology (IMCB), A*STAR, A*STAR JCO Career Development Award (CDA) 15302FG148, NMRC/OFYIRG/018/2016-00, A*STAR ETPL Gap Funding ETPL/18-GAP005-R20H, Lee Foundation Grant SHTX/LFG/002/2018, Skin Innovation Grant SIG18011, NMRC OF-LCG/DYNAMO, FY2019 SingHealth Duke-NUS Surgery Academic Clinical Programme Research Support Programme Grant, Precision Medicine and Personalised Therapeutics Joint Research Grant 2019, SingHealth Duke-NUS Transplant Centre - Multi-Visceral Transplant Fund Award and the Industry Alignment Fund - Industry Collaboration Project (IAF-ICP). This work was also supported by a National Institutes of Health Grant R01 DK67536, the Harvard Stem Cell Institute (R.N.K.), NIH/NIDDK grant K01 DK098285 (J.A.P.), Harvard Stem Cell Institute (HSCI) and a U.S. National Institutes of Health (NIH) F31DK098931 (I.A.V), Western Norway Regional Health Authority, Diabetesforbundet, Bergen Research Foundation, and the Novo Nordisk Foundation (H.R.), Norsk Endokrinologisk forenings reisestipend, Diabetesforbundet and Inger R. Haldorsens legat (H.V.).
The full paper is available at the publisher's URL here: https://doi.org/10.1002/stem.3135
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