Cellular Subcompartments through Cytoplasmic Streaming Pieuchot, Laurent et al. Developmental Cell, Volume 34, Issue 4, 410 - 420
Abstract:
Cytoplasmic streaming occurs in diverse cell types, where it generally serves a transport function. Here, we examine streaming in multicellular fungal hyphae and identify an additional function wherein regimented streaming forms distinct cytoplasmic subcompartments. In the hypha, cytoplasm flows directionally from cell to cell through septal pores. Using live-cell imaging and computer simulations, we identify a flow pattern that produces vortices (eddies) on the upstream side of the septum. Nuclei can be immobilized in these microfluidic eddies, where they form multinucleate aggregates and accumulate foci of the HDA-2 histone deacetylase-associated factor, SPA-19. Pores experiencing flow degenerate in the absence of SPA-19, suggesting that eddy-trapped nuclei function to reinforce the septum. Together, our data show that eddies comprise a subcellular niche favoring nuclear differentiation and that subcompartments can be self-organized as a consequence of regimented cytoplasmic streaming.
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Funding Info:
We gratefully acknowledge the use of deletion mutants generated by NIH grant P01 GM068087 “Functional Analysis of a Model Filamentous Fungus.” F.Y.L. and K.-H.C. are supported by the Agency for Science, Technology and Research (A∗STAR), Singapore. Research in the Jedd Group is supported by the Temasek Life Sciences Laboratory.
Description:
The full paper can be downloaded from the publisher's URL here: https://doi.org/1016/j.devcel.2015.07.017