Aw, Jong Ghut Ashley et al. Molecular Cell , Volume 62 , Issue 4 , 603 - 617
Abstract:
Identifying pairwise RNA-RNA interactions is key to understanding how RNAs fold and interact with other RNAs inside the cell. We present a high-throughput approach, sequencing of psoralen crosslinked, ligated, and selected hybrids (SPLASH), that maps pairwise RNA interactions in vivo with high sensitivity and specificity, genome-wide. Applying SPLASH to human and yeast transcriptomes revealed the diversity and dynamics of thousands of long-range intra- and intermolecular RNA-RNA interactions. Our analysis highlighted key structural features of RNA classes, including the modular organization of mRNAs, its impact on translation and decay, and the enrichment of long-range interactions in noncoding RNAs. Additionally, intermolecular mRNA interactions were organized into network clusters and were remodeled during cellular differentiation. We also identified hundreds of known and new snoRNA-rRNA binding sites, expanding our knowledge of rRNA biogenesis. These results highlight the underexplored complexity of RNA interactomes and pave the way to better understanding how RNA organization impacts biology.
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Funding Info:
A.Y.L.S. is supported by the A*STAR Joint Council Office Career Development Award (project number 15302FG145). N.N. is supported by funding from A*STAR. Y.W. is supported by funding from A*STAR and Society in Science- Branco Weiss Fellowship.
Description:
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