OmpA: A Flexible Clamp for Bacterial Cell Wall Attachment

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OmpA: A Flexible Clamp for Bacterial Cell Wall Attachment
Title:
OmpA: A Flexible Clamp for Bacterial Cell Wall Attachment
Journal Title:
Structure
Publication URL:
Keywords:
Publication Date:
06 December 2016
Citation:
OmpA: A Flexible Clamp for Bacterial Cell Wall Attachment Samsudin, Firdaus et al. Structure , Volume 24 , Issue 12 , 2227 - 2235
Abstract:
The envelope of Gram-negative bacteria is highly complex, containing separate outer and inner membranes and an intervening periplasmic space encompassing a peptidoglycan (PGN) cell wall. The PGN scaffold is anchored non-covalently to the outer membrane via globular OmpA-like domains of various proteins. We report atomically detailed simulations of PGN bound to OmpA in three different states, including the isolated C-terminal domain (CTD), the full-length monomer, or the complete full-length dimeric form. Comparative analysis of dynamics of OmpA CTD from different bacteria helped to identify a conserved PGN-binding mode. The dynamics of full-length OmpA, embedded within a realistic representation of the outer membrane containing full-rough (Ra) lipopolysaccharide, phospholipids, and cardiolipin, suggested how the protein may provide flexible mechanical support to the cell wall. An accurate model of the heterogeneous bacterial cell envelope should facilitate future efforts to develop antibacterial agents.
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PublisherCopyrights
Funding Info:
This work was funded by the Biotechnology and Biological Sciences Council (grant no. BB/M029573/1) and the Institute for Life Sciences, University of Southampton. This project made use of time on ARCHER granted via the UK High-End Computing Consortium for Biomolecular Simulation, HECBioSim (www.hecbiosim.ac.uk), supported by EPSRC (grant no. EP/L000253/1).
Description:
Full paper can be downloaded from the Publisher's URL provided.
ISSN:
0969-2126
1878-4186
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