A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection

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A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection
Title:
A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection
Other Titles:
Sensors
Publication Date:
16 October 2017
Citation:
Wong, C.L.; Chua, M.; Mittman, H.; Choo, L.X.; Lim, H.Q.; Olivo, M. A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection. Sensors 2017, 17, 2363.
Abstract:
In this paper, we present a phase-intensity surface plasmon resonance (SPR) biosensor and demonstrate its use for avian influenza A (H5N1) antibody biomarker detection. The sensor probes the intensity variation produced by the steep phase response at surface plasmon excitation. The prism sensor head is fixed between a pair of polarizers with a perpendicular orientation angle and a forbidden transmission path. At SPR, a steep phase change is introduced between the p- and s-polarized light, and this rotates the polarization ellipse of the transmission beam. This allows the light at resonance to be transmitted and a corresponding intensity change to be detected. Neither time-consuming interference fringe analysis nor a phase extraction process is required. In refractive index sensing experiments, the sensor resolution was determined to be 6.3 × 10-6 refractive index values (RIU). The sensor has been further applied for H5N1 antibody biomarker detection, and the sensor resolution was determined to be 193.3 ng mL-1, compared to 1 μg mL-1 and 0.5 μg mL-1, as reported in literature for influenza antibody detection using commercial Biacore systems. It represents a 517.3% and 258.7% improvement in detection limit, respectively. With the unique features of label-free, real-time, and sensitive detection, the phase-intensity SPR biosensor has promising potential applications in influenza detection.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
Description:
ISSN:
1424-8220
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