Phenotypic screening for inhibitors of a mutant thrombopoietin receptor

Phenotypic screening for inhibitors of a mutant thrombopoietin receptor
Title:
Phenotypic screening for inhibitors of a mutant thrombopoietin receptor
Other Titles:
Methods in Molecular Biology
DOI:
10.1007/978-1-4939-7847-2_4
Publication Date:
08 May 2018
Citation:
Ngo A. et al. (2018) Phenotypic Screening for Inhibitors of a Mutant Thrombopoietin Receptor. In: Wagner B. (eds) Phenotypic Screening. Methods in Molecular Biology, vol 1787. Humana Press, New York, NY
Abstract:
An inhibitor for the thrombopoietin receptor (TpoR) would be more specific for the treatment of myeloproliferative neoplasms (MPNs) due to constitutively active mutant TpoR compared to the current treatment approach of inhibiting Janus kinase 2 (JAK2). We describe a cell-based high throughput phenotypic screening approach to identify inhibitors for constitutively active mutant TpoR. A stepwise elimination process is used to differentiate generally cytotoxic compounds from compounds that specifically inhibit growth of cells expressing wild type TpoR and/or mutant TpoR. We have systematically optimized the phenotypic screening assay and documented in this article critical parameters for a successful phenotypic screen, such as cell growth and seeding optimization, plate reproducibility and uniformity studies, and an assay robustness analysis with a pilot screen.
License type:
PublisherCopyrights
Funding Info:
Support for A.N., A.K. and M.L.C was from an A*STAR core funding to the Experimental Therapeutics Centre. Support for C.C.D. was from UEFISCDI PCCA PN II 133/2012 and OPERATIONAL COMPETITIVITATY PROGRAM 2014-2020 POC-A1-A1.1.4-E-2015/ P_37_798/ 149/2016. S.N.C. and A.K.S. receive funding from the Ludwig Institute for Cancer Research. S.N.C. also received supports from FRS-FNRS, Salus Sanguinis Foundation, the Action de Recherche Concertée project ARC10/15–027 of the Université catholique de Louvain, the Fondation contre le Cancer, the PAI Programs BCHM61B5 and Belgian Medical Genetics Initiative.
Description:
ISSN:
978-1-4939-7847-2
ISBN:
978-1-4939-7846-5
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