Structural and ligand binding analysis of the YAP-binding domain of transcription factor TEAD4

Structural and ligand binding analysis of the YAP-binding domain of transcription factor TEAD4
Title:
Structural and ligand binding analysis of the YAP-binding domain of transcription factor TEAD4
Other Titles:
Biochemical Journal
Publication Date:
23 May 2018
Citation:
Biochemical Journal May 14, 2018, BCJ20180225;
Abstract:
The oncoprotein YAP requires the TEAD family of transcription factors for the upregulation of genes important for cell proliferation. Disrupting YAP-TEAD interaction is an attractive strategy for cancer therapy. Targeting TEADs using small molecules that either bind to the YAP-binding pocket or the palmitate-binding pocket is proposed to the disrupt YAP-TEAD interaction. There is a need for methodologies to facilitate robust and reliable identification of compounds that occupy either YAP-binding pocket or palmitate-binding pocket. Here using NMR spectroscopy, we validated compounds that bind to these pockets and also identify the residues in mouse TEAD4 (mTEAD4) that interact with these compounds. Flufenamic acid (FA) was used as positive control for validation of palmitate-binding pocket-occupying compounds by NMR. Further, we identify a hit from a fragment screen and show that it occupies a site close to YAP-binding pocket on the TEAD surface. Our results also indicate that purified mTEAD4 can catalyze auto-palmitoylation. NMR studies on mTEAD4 revealed that exchanges exist in TEAD as NMR signal broadening was observed for residues close to the palmitoylation site. Mutating the palmitoylated cysteine (C360S mutant) abolished palmitoylation while no significant changes in the NMR spectrum were observed for the mutant which still binds to YAP. We also show that FA inhibits TEAD auto-palmitoylation. Our studies highlight the utility of NMR spectroscopy in identifying small molecules that bind to TEADs pockets and reinforces the notion that both palmitate-binding pocket and YAP-binding pocket are targetable.
License type:
PublisherCopyrights
Funding Info:
A*STAR JCO grant (1431AFG102/1331A028)
Description:
ISSN:
0264-6021
1470-8728
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