B. Kim, S. Feng, S.-W. Yun, C. Leong, R. Satapathy, S. Y. D. Wan, Y.-T. Chang, ChemBioChem 2016, 17, 2118.
Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2low cells from heterogeneous cell populations.
This is the peer reviewed version of the following article: B. Kim, S. Feng, S.-W. Yun, C. Leong, R. Satapathy, S. Y. D. Wan, Y.-T. Chang, ChemBioChem 2016, 17, 2118, which has been published in final form at http://dx.doi.org10.1002/cbic.201600490. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving