Quantitative assessment of telomerase components in cancer cell lines

Quantitative assessment of telomerase components in cancer cell lines
Title:
Quantitative assessment of telomerase components in cancer cell lines
Other Titles:
FEBS Letters
Keywords:
Publication Date:
05 March 2015
Citation:
Akıncılar Semih Can,Low Kee Chung,Liu Chia Yi,Yan Ting Dong,Oji Asami,Ikawa Masahito,Li Shang and Tergaonkar Vinay(2015), Quantitative assessment of telomerase components in cancer cell lines, FEBS Letters, 589, doi: 10.1016/j.febslet.2015.02.035
Abstract:
Besides its canonical function of catalyzing the formation of telomeric repeats, many groups have recently reported non-canonical functions of hTERT in particular, and telomerase in general. Regulating transcription is the central basis of non-canonical functions of telomerase. However, unlike reverse transcriptase activity of telomerase that requires only a few molecules of enzymatically active hTERT, non-canonical functions of hTERT or other telomerase components theoretically require several hundred copies. Here, we provide the first direct quantification of all the telomerase components in human cancer cell lines. We demonstrate that telomerase components do not exist in a 1:1 stoichiometric ratio, and there are several hundred copies of hTERT in cells. This provides the molecular basis of hTERT to function in other signaling cascades, including transcription.
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Full paper can be downloaded from the Publisher's URL provided.
ISSN:
0014-5793
1873-3468
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