A resource of ribosomal RNA-depleted RNA-Seq data from different normal adult and fetal human tissues

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A resource of ribosomal RNA-depleted RNA-Seq data from different normal adult and fetal human tissues
Title:
A resource of ribosomal RNA-depleted RNA-Seq data from different normal adult and fetal human tissues
Journal Title:
Scientific Data
Keywords:
Publication Date:
10 November 2015
Citation:
Scientific Data 2, Article number: 150063 (2015) doi:10.1038/sdata.2015.63
Abstract:
Gene expression is the most fundamental level at which the genotype leads to the phenotype of the organism. Enabled by ultra-high-throughput next-generation DNA sequencing, RNA-Seq involves shotgun sequencing of fragmented RNA transcripts by next-generation sequencing followed by in silico assembly, and is rapidly becoming the most popular method for gene expression analysis. Poly[A]+ RNA-Seq analyses of normal human adult tissue samples such as Illumina’s Human BodyMap 2.0 Project and the RNA-Seq atlas have provided a useful global resource and framework for comparisons with diseased tissues such as cancer. However, these analyses have failed to provide information on poly[A]−RNA, which is abundant in our cells. The most recent advances in RNA-Seq analyses use ribosomal RNA-depletion to provide information on both poly[A]+ and poly[A]−RNA. In this paper, we describe the use of Illumina’s HiSeq 2000 to generate high quality rRNA-depleted RNA-Seq datasets from human fetal and adult tissues. The datasets reported here will be useful in understanding the different expression profiles in different tissues.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
Description:
ISSN:
2052-4463
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