Lgr5 Marks Stem/Progenitor Cells in Ovary and Tubal Epithelia

Lgr5 Marks Stem/Progenitor Cells in Ovary and Tubal Epithelia
Title:
Lgr5 Marks Stem/Progenitor Cells in Ovary and Tubal Epithelia
Other Titles:
Nature Cell Biology
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Publication Date:
06 July 2014
Citation:
Nature Cell Biology 16, 745–757 (2014)
Abstract:
The ovary surface epithelium (OSE) undergoes ovulatory tear and remodelling throughout life. Resident stem cells drive such tissue homeostasis in many adult epithelia, but their existence in the ovary has not been definitively proven. ​Lgr5 marks stem cells in multiple epithelia. Here we use reporter mice and single-molecule fluorescent in situ hybridization to document candidate ​Lgr5+ stem cells in the mouse ovary and associated structures. ​Lgr5 is broadly expressed during ovary organogenesis, but becomes limited to the OSE in neonate life. In adults, ​Lgr5 expression is predominantly restricted to proliferative regions of the OSE and mesovarian–fimbria junctional epithelia. Using in vivo lineage tracing, we identify embryonic and neonate ​Lgr5+ populations as stem/progenitor cells contributing to the development of the OSE cell lineage, as well as epithelia of the mesovarian ligament and oviduct/fimbria. Adult ​Lgr5+ populations maintain OSE homeostasis and ovulatory regenerative repair in vivo. Thus, ​Lgr5 marks stem/progenitor cells of the ovary and tubal epithelia.
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PublisherCopyrights
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ISSN:
1465-7392
1476-4679
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