Human Finger-Prick Induced Pluripotent Stem Cells Facilitate the Development of Stem Cell Banking. Hong-Kee Tan, Cheng-Xu Delon Toh, Dongrui Ma, Binxia Yang, Tong Ming Liu, Jun Lu, Chee-Wai Wong, Tze-Kai Tan, Hu Li, Christopher Syn, Eng-Lee Tan, Bing Lim, Yoon-Pin Lim, Stuart A. Cook, Yuin-Han Loh. Stem Cells Trans Med 2014; 3:586-598; first published on March 19, 2014; doi:10.5966/sctm.2013-0195
Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients can be a good model for studying human diseases and for future therapeutic regenerative medicine. Current initiatives to establish human iPSC (hiPSC) banking face challenges in recruiting large numbers of donors with diverse diseased, genetic, and phenotypic representations. In this study, we describe the efficient derivation of transgene-free hiPSCs from human finger-prick blood. Finger-prick sample collection can be performed on a "do-it-yourself" basis by donors and sent to the hiPSC facility for reprogramming. We show that single-drop volumes of finger-prick samples are sufficient for performing cellular reprogramming, DNA sequencing, and blood serotyping in parallel. Our novel strategy has the potential to facilitate the development of large-scale hiPSC banking worldwide.
J.L. is supported by the National Research Foundation (NRF2008-CRP001-68) of Singapore and a Duke-NUS GOH Cardiovascular Research Award (Duke-NUS-GCR/2012/0005R). H.L. is supported by Mayo Clinic Center for Individualized Medicine. Y.-H.L. is supported by the A*STAR Investigatorship research award. We are grateful to the Biomedical Research Council, Agency for Science, Technology, and Research, Singapore, for research funding.
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