Selection of suitable detergents for obtaining an active dengue protease in its natural form from E. coli

Selection of suitable detergents for obtaining an active dengue protease in its natural form from E. coli
Title:
Selection of suitable detergents for obtaining an active dengue protease in its natural form from E. coli
Other Titles:
Protein Expression and Purification
Publication Date:
03 February 2016
Citation:
Lynette Sin Yee Liew, Michelle Yueqi Lee, Ying Lei Wong, Jinting Cheng, Qingxin Li, CongBao Kang, Selection of suitable detergents for obtaining an active dengue protease in its natural form from E. coli, Protein Expression and Purification, Volume 121, May 2016, Pages 141-148, ISSN 1046-5928, http://dx.doi.org/10.1016/j.pep.2016.02.001.
Abstract:
Dengue protease is a two-component enzyme and is an important drug target against dengue virus. The protease activity and protein stability of dengue nonstructural protein 3 (NS3) require a co-factor region of a four-span membrane protein NS2B. A natural form of dengue protease containing full-length NS2B and NS3 protease domain NS2BFL-NS3pro is useful for dengue drug discovery. In current study, detergents that can be used for protease purification were tested. Using a water soluble protease construct, 39 detergents were selected for NS2B and NS2BFL-NS3pro purification. The results show that 18 detergents were able to sustain the activity of the natural dengue protease and 11 detergents could be used for NS2B purification. The results obtained in this study will be useful for biochemical and biophysical studies on dengue protease.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
A*STAR JCO grants (1331A028) and the Biospecialties project (1526004161).
Description:
ISSN:
1046-5928
1096-0279
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