Michelle Meng Huang Mok, Linsen Du, Chelsia Qiuxia Wang, Vinay Tergaonkar, Te Chih Liu, Shirley Kow Yin Kham, Takaomi Sanda, Allen Eng-Juh Yeoh, Motomi Osato, RUNX1 point mutations potentially identify a subset of early immature T-cell acute lymphoblastic leukaemia that may originate from differentiated T-cells, Gene, Volume 545, Issue 1, 15 July 2014, Pages 111-116, ISSN 0378-1119, http://dx.doi.org/10.1016/j.gene.2014.04.074. (http://www.sciencedirect.com/science/article/pii/S0378111914005162)
The RUNX1/AML1 gene is among the most frequently mutated genes in human leukaemia. However, its association with T-cell acute lymphoblastic leukaemia (T-ALL) remains poorly understood. In order to examine RUNX1 point mutations in T-ALL, we conducted an amplicon-based deep sequencing in 65 Southeast Asian childhood patients and 20 T-ALL cell lines, and detected RUNX1 mutations in 6 patients (9.2%) and 5 cell lines (25%). Interestingly, RUNX1-mutated T-ALL cases seem to constitute a subset of early immature T-ALL that may originate from differentiated T-cells. This result provides a deeper insight into the mechanistic basis for leukaemogenesis.