The small GTPase Arf1 modulates mitochondrial morphology and function

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The small GTPase Arf1 modulates mitochondrial morphology and function
Title:
The small GTPase Arf1 modulates mitochondrial morphology and function
Journal Title:
The EMBO Journal
Keywords:
Publication Date:
04 September 2014
Citation:
The EMBO Journal (2014) 33, 2659-2675
Abstract:
The small GTPase Arf1 plays critical roles in membrane traffic by initiating the recruitment of coat proteins and by modulating the activity of lipid‐modifying enzymes. Here, we report an unexpected but evolutionarily conserved role for Arf1 and the ArfGEF GBF1 at mitochondria. Loss of function of ARF‐1 or GBF‐1 impaired mitochondrial morphology and activity in Caenorhabditis elegans. Similarly, mitochondrial defects were observed in mammalian and yeast cells. In Saccharomyces cerevisiae, aberrant clusters of the mitofusin Fzo1 accumulated in arf1‐11 mutants and were resolved by overexpression of Cdc48, an AAA‐ATPase involved in ER and mitochondria‐associated degradation processes. Yeast Arf1 co‐fractionated with ER and mitochondrial membranes and interacted genetically with the contact site component Gem1. Furthermore, similar mitochondrial abnormalities resulted from knockdown of either GBF‐1 or contact site components in worms, suggesting that the role of Arf1 in mitochondrial functioning is linked to ER–mitochondrial contacts. Thus, Arf1 is involved in mitochondrial homeostasis and dynamics, independent of its role in vesicular traffic.
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PublisherCopyrights
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Full paper can be downloaded from the Publisher's URL provided.
ISSN:
0261-4189
1460-2075
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